108. BEGGIATOA MEDIUM 2

MgCl₂ × 6 H₂O 50.0 mg

CaCl₂ × 2 H₂O 30.0 mg

Na-lactate 500.0 mg

Na₂S₂O₃ × 5 H₂O 500.0 mg

NaH₂PO₄ 125.0 mg

KCl 125.0 mg

NaHCO₃ 125.0 mg

Na₂SO₄ 500.0 mg

NaNO₃ 620.0 mg

Vitamin solution (see below) 1.0 ml

Trace element solution according to Hogland: (see below) 1.0 ml

Distilled water 1000.0 ml

Vitamin solution:

Biotin 200.0 mg

Folic acid 20.0 mg

Pyridoxine-HCl 100.0 mg

Thiamine-HCl 50.0 mg

Riboflavin 100.0 mg

Nicotinic acid 50.0 mg

DL-Pantothenic acid 50.0 mg

Vitamin B₁₂ 1.0 mg

p-Aminobenzoic acid 50.0 mg

Distilled water 1000.0 ml

Trace element solution according to Hogland:

EDTA 5.0 g

FeSO₄ × 7 H₂O 2.0 g

ZnSO₄ × 7 H₂O 100.0 mg

MnCl₂ × 4 H₂O 30.0 mg

H₃BO₃ 300.0 mg

CoCl₂ × 6 H₂O 200.0 mg

CuCl₂ 10.0 mg

NiCl₂ × 6 H₂O 20.0 mg

Na₂MoO₄ × 2 H₂O 20.0 mg

Distilled water 1000.0 ml

pH 7.5 (adjust with 1% HCl)

To prepare the trace element solution, preliminarily acidify water to pH 3.0-4.0 with HCl. Sterilize thiosulfate, lactate, bicarbonate, trace elements and vitamins separately and add to the main medium prior to inoculation. Lactate and thiosulfate can be more conveniently prepared as 10% solutions; bicarbonate, as 5% solution. Sterilize the vitamin solution by filtration, others solutions and base medium by autoclaving at 121°C for 15 min.

 

Updated 28.05.2023