110. DESULFOVIBRIO MEDIUM WITH 2% NaCl

Solution 1:

K₂HPO₄ 0.5 g

NH₄Cl 1.0 g

NaCl 20 g

Na₂SO₄ 1.0 g

CaCl₂ × 2 H₂O 0.1 g

MgSO₄ × 7 H₂O 2.0 g

Na DL-lactate 2.0 g

Yeast extract 1.0 g

Resazurin 1.0 mg

Distilled water 980.0 ml

Solution 2:

FeSO₄ × 7 H₂O 0.5 g

Distilled water 10.0 ml

Solution 3:

Na-thioglycollate 0.1 g

Ascorbic acid 0.1 g

Distilled water 10.0 ml

Solution 4:

Trace element solution (see below) 1.0 ml

Trace element solution:

FeCl₂ × 4 H₂O 1.5 g

ZnCl₂ 68.0 mg

MnCl₂ × 4 H₂O 100.0 mg

H₃BO₃ 62.0 mg

CoCl₂ × 6 H₂O 120.0 mg

Na₂MoO₄ × 2 H₂O 24.0 mg

HCl, 0.05 M 1000.0 ml

pH 7.4-7.8

Autoclave at 121°C for 15 min under an atmosphere of N₂. Solution 1 is boiled before sterilization, being blown with N₂.

 

Updated 28.05.2023