124. AZOSPIRILLUM MEDIUM

Solution 1:

Yeast extract 0.05 g

FeSO₄ × 7 H₂O 0.01 g

K₂HPO₄ 0.25 g

Na₂MoO₄ × 2 H₂O 1.0 mg

MnSO₄ × H₂O 2.0 mg

MgSO₄ × 7 H₂O 0.2 g

NaCl 0.1 g

CaCl₂ × 2 H₂O 0.02 g

(NH₄)₂SO₄ 1.0 g

Biotin 0.1 mg

Bromothymol blue 25.0 mg

Distilled water 950.0 ml

Solution 2:

Glucose 20% 25.0 ml

Solution 3:

Na-malate 20% 25.0 ml

Dissolve bromothymol blue in diluted KOH before adding into the medium.

pH 7.1

Autoclave solutions separately at 121°C for 15 min.

 

Updated 01.02.2024