128. HIRSCH MEDIUM

KH₂PO₄ 1.36 g

Na₂HPO₄ 2.15 g

MgSO₄ × 7 H₂O 0.2 g

MnSO₄ × 5 H₂O 1.05 g

(NH₄)₂SO₄ 0.5 g

CaCl₂ × 2 H₂O 5.97 mg

Na₂MoO₄ × 2 H₂O 1.5 mg

Methanol 5.0 ml

Distilled water to 1000.0 ml

pH 7.0

Autoclave at 121°C for 15 min. Sterilize methanol by filtration and add to the medium after autoclaving.

 

Updated 27.05.2023