131. MEDIUM Q MOD FOR FRANKIA

K₂HPO₄ 300.0 mg

NaH₂PO₄ 200.0 mg

MgSO₄ × 7 H₂O 200.0 mg

KCl 200.0 mg

Yeast extract 500.0 mg

Peptone 5.0 g

Glucose 10.0 g

Fe-citrate 1.0 ml

Trace element solution (see below) 1.0 ml

CaCO₃ 100.0 mg

Tween-80 2.0 g

Agar 15.0 g

Distilled water 1000.0 ml

Trace element solution:

H₃BO₃ 1.5 g

MnSO₄ × 7 H₂O 0.8 g

ZnSO₄ × 7 H₂O 0.6 g

CuSO₄ × 5 H₂O 0.1 g

(NH₄)₆Mo₇O₂₄ × 4 H₂O 0.2 g

CoSO₄ 0.001 g

Distilled water 1000.0 ml

pH 7.0

Autoclave at 111°C for 30 min.

 

Updated 21.06.2021