133. COLBY AND ZATMAN MEDIUM WITH METHANOL

K₂HPO₄ 1.2 g

KH₂PO₄ 0.62 g

CaCl₂ × 6 H₂O 0.05 g

MgSO₄ × 7 H₂O 0.2 g

NaCl 0.1 g

FeCl₃ × 6 H₂O 1.0 mg

(NH₄)₂SO₄ 0.5 g

CuSO₄ × 5 H₂O 5.0 µg

MnSO₄ × 5 H₂O 10.0 µg

Na₂MoO₄ × 2 H₂O 10.0µg

H₃BO₃ 10.0 µg

ZnSO₄ × 7 H₂O 70.0 µg

CoCl₂ × 6 H₂O 5.0µg

Purified agar 15.0 g

Methanol 1.0 ml

Distilled water 1000.0 ml

pH 6.8

Autoclave at 121°C for 15 min. Methanol is filter sterilized.

 

Updated 01.02.2024