140. DILUTED BRAIN HEART INFUSION MEDIUM (DILUTED BHI MEDIUM)

BHI broth (Pronadisa 1400) 3.7 g or

Peptone mixture 1.0 g

Beef heart infusion 1.0 g

Calf brain infusion 0.75 g

Glucose 0.2 g

Na₂HPO₄ 0.25 g

NaCl 0.5 g

Agar 15.0 g

Distilled water 1000.0 ml

pH 7.4±0.2

Sterilize at 121°C for 15 min.