142. PPYA

Potato decoction (see below) 200.0 ml

Peptone 5.0 g

Yeast extract 1.0 g

Agar 25.0 g

Distilled water to 1000.0 ml

pH 8.0

Sterilize at 121°C for 15 min

Preparation of potato decoction:

Boil 200 g scrubbed and sliced potatoes in 1000.0 ml water for 1 hour, filter cold through a cotton-gauze filter, add water to the initial volume, add glucose and agar. Do not use new potatoes.

 

Updated 09.08.2021