153. MEDIUM P-2 FOR THERMOPHILIC ANAEROBIC BACTERIA

K₂HPO₄ 3.0 g

KH₂PO₄ 2.0 g

NH₄Cl 2.0 g

MgCl₂ × 6 H₂O 0.2 g

CaCl₂ × 2 H₂O 0.05 g

Tryptone 10.0 g

Glucose 5.0 g

Yeast extract 5.0 g

Resazurin 0.01 g

Distilled water 1000.0 ml

pH 7.0-7.2

Prepare medium anaerobically under N₂ and autoclave (удалить under N₂ at 121°C for 15 min. Autoclave glucose separately under N₂ at 111°C for 30 min.

 

Updated 1.02.2024