180. DESULFOVIBRIO CARBINOLICUS MEDIUM

Solution 1:

Na₂SO₄ 3.0 g

KH₂PO₄ 0.2 g

NH₄Cl 0.3 g

NaCl 1.0 g

MgCl₂ × 6 H₂O 0.4 g

KCl 0.5 g

CaCl₂ × 2 H₂O 0.15 g

Distilled water 870.0 ml

Solution 2:

Trace element solution SL-10 (see below) 1.0 ml

Solution 3:

NaHCO₃ 5.0 g

Distilled water 100.0 ml

Solution 4:

Ethanol 0.7 g

Casamino acids 0.1 g

Yeast extract 0.1 g

Distilled water 10.0 ml

Solution 5:

Vitamin solution (see below) 10.0 ml

Solution 6:

Na₂S × 9 H₂O 0.4 g

Distilled water 10.0 ml

Trace element solution SL-10:

HCl (25%; 7.7 M) 10.0 ml

FeCl₂ × 4 H₂O 1.5 g

ZnCl₂ 70.0 mg

MnCl₂ × 4 H₂O 100.0 mg

H₃BO₃ 6.0 mg

CoCl₂ × 6 H₂O 190.0 mg

CuCl₂ × 2 H₂O 2.0 mg

NiCl₂ × 6 H₂O 24.0 mg

Na₂MoO₄ × 2 H₂O 36.0 mg

Distilled water 990.0 ml

Vitamin solution:

Biotin 2.0 mg

Folic acid 2.0 mg

Pyridoxine-HCl 10.0 mg

Thiamine-HCl 5.0 mg

Riboflavin 5.0 mg

Nicotinic acid 5.0 mg

Ca DL-pantothenate 5.0 mg

Vitamin B₁₂ 0.1 mg

p-Aminobenzoic acid 5.0 mg

Lipoic acid 5.0 mg

Distilled water 1000.0 ml

Solution 1 is boiled before sterilization for a few minutes being flushed with gas mixture of 80% N₂ and 20% CO₂ with sodium bicarbonate added until an equilibrium pH of 6.9-7.1. Solution 1 is autoclaved under this gas mixture at 121°C for 15 min. Solution 2 is autoclaved under 100% N₂ at 121°C for 15 min. Vitamin solution is filter sterilized. Others solutions autoclave at 121°C for 15 min and add to base medium aseptically.

Final pH of the complete medium 7.1-7.4

 

Updated 1.02.2024