188. Natronobacterium MEDIUM

Solution 1:

KH₂PO₄ 1.0 g

KCl 1.0 g

NH₄Cl 1.0 g

MgSO₄ × 7 H₂O 0.24 g

CaSO₄ × 2 H₂O 0.17 g

Trace element solution SL-10 (see below) 1.0 ml

NaCl 200.0 g

Glutamate 1.0 g

Yeast extract 5.0 g

Casamino acids 5.0 g

Agar, if necessary (heat and dissolve it before adding sodium chloride) 20.0 g

Distilled water to 1000.0 ml

pH 6.5

Solution 2:

Na₂CO₃ 5.0 g

Distilled water 50.0 ml

Trace element solution SL-10:

HCl (25%; 7.7 M) 10.0 ml

FeCl₂ × 4 H₂O 1.5 g

ZnCl₂ 70.0 mg

MnCl₂ × 4 H₂O 100.0 mg

H₃BO₃ 6.0 mg

CoCl₂ × 6 H₂O 190.0 mg

CuCl₂ × 2 H₂O 2.0 mg

NiCl₂ × 6 H₂O 24.0 mg

Na₂MoO₄ × 2 H₂O 36.0 mg

Distilled water 990.0 ml

pH 9.0-9.5

Autoclave separately at 121°C for 15 min and mix aseptically.

 

Updated 1.02.2024