197. MEDIUM FOR RHODOSPIRILLUM (PFENNIG MEDIUM)

NH₄Cl 0.4 g

KH₂PO₄ 0.5 g

MgSO₄ × 7 H₂O 0.2 g

NaCl 0.4 g

CaCl₂ × 2 H₂O 0.05 g

Acetate, or butyrate, or propionate, or succinate 1.0 g

Yeast extract 0.2 g

Fe-citrate (0.1%) 5.0 ml

Trace element solution according to Pfennig (see below) 1.0 ml

Vitamin B₁₂ (dispensary solution, 0,01%) 1.0 ml

Distilled water 1000.0 ml

Trace element solution according to Pfennig:

EDTA 1.5 g

Trace element solution according to Hogland (see below) 6.0 ml

FeSO₄ × 7 H₂O 0.2 g

MnCl₂ × 4 H₂O 0.02 g

ZnSO₄ × 7 H₂O 0.1 g

Distilled water 1000.0 ml

Trace element solution according to Hogland:

EDTA 5.0 g

FeSO₄ × 7 H₂O 2.0 g

ZnSO₄ × 7 H₂O 100.0 mg

MnCl₂ × 4 H₂O 30.0 mg

H₃BO₃ 300.0 mg

CoCl₂ × 6 H₂O 200.0 mg

CuCl₂ × 2 H₂O 10.0 mg

NiCl₂ × 6 H₂O 20.0 mg

Na₂MoO₄ × 2 H₂O 20.0 mg

Distilled water 1000.0 ml

Autoclave base medium and trace element solution separately at 121°C for 15 min.

 

Updated 1.02.2024