204. MEDIUM FOR CLOSTRIDIUM

Glucose 10.0 g

Peptone 12.0 g

NaCl 2.0 g

Agar 16.0 g

Distilled water 1000.0 ml

The medium can be used with chalk addition as buffer against acidification of the medium during cultivation.

pH 6.8-7.2

Sterilize at 111 C for 30 min.