206. ISP MEDIUM 3

Oatmeal 20.0 g

Salt solution A (see below) 1.0 ml

Agar 20.0 g

Distilled water 1000.0 ml

Salt solution A:

FeSO₄ × 7 H₂O 0.1 g

MnCl₂ × 4 H₂O 0.1 g

ZnSO₄ × 7 H₂O 0.1 g

Distilled water 100.0 ml

Boil oat flakes in water bath for 20 min filter through a cotton-gauze filter, fill up to 1000.0 ml.

pH 7.2

Autoclave separately at 121°C 15 min.

 

Updated 1.02.2024