234. Desulfohalobium utahense medium

NaCl 100.0 g

MgSO₄ × 7 H₂O 10.0 g

KCl 6.0 g

CaCl₂ × 2 H₂O 0.4 g

NH₄Cl 1.0 g

KH₂PO₄ 0.1 g

Yeast extract 0.5 g

Trace element solution SL-10 (see below) 1.0 ml

Selenite-tungstate solution (see below) 1.0 ml

NaHCO₃ 4.0 g

Na-(L)-lactate 2.5 g

Resazurin 0.5 mg

Na₂S × 9 H₂O 0.3 g

Distilled water 1000.0 ml

Trace element solution SL-10:

HCl (25%; 7.7 M) 10.0 ml

FeCl₂ × 4 H₂O 1.5 g

ZnCl₂ 70.0 mg

MnCl₂ × 4 H₂O 100.0 mg

H₃BO₃ 6.0 mg

CoCl₂ × 6 H₂O 190.0 mg

CuCl₂ × 2 H₂O 2.0 mg

NiCl₂ × 6 H₂O 24.0 mg

Na₂MoO₄ × 2 H₂O 36.0 mg

Distilled water 990.0 ml

Selenite-tungstate solution:

NaOH 0.5 g

Na₂SeO₃ × 5 H₂O 3 mg

Na₂WO₄ × 2 H₂O 4 mg

Distilled water 1000.0 ml

Dissolve ingredients (except lactate, bicarbonate and sulfide), boil medium for 1 min, then cool to room temperature under 80% N₂ and 20% CO₂ gas mixture. Dispense under same gas atmosphere in culture vessels and autoclave at 121°C 15 min. Add sodium lactate and sulfide from sterile anoxic stock solutions prepared under N₂ and bicarbonate from a sterile stock solution prepared under 80% N₂ and 20% CO₂ (all solutions sterilize separately at 121°C 15 min).

Final pH of the medium 7.0-7.2

 

Updated 1.02.2024