235. Desulfonatronum cooperativum medium

KH₂PO₄ 0.2 g

MgCl₂ × 6 H₂O 0.1 g

NH₄Cl 1.0 g

KCl 0.2 g

Na₂SO₄ 5.0 g

NaCl 10.0 g

Na₂CO₃ 3.5 g

Trace element solution SL-10 (see below) 1.0 ml

Vitamin solution (see below) 10.0 ml

Yeast extract 1.0 g

Resazurin 0.5 mg

Na-formate 4.0 g

Na₂S × 9 H₂O 0.5 g

Distilled water 1000.0 ml

Trace element solution SL-10:

HCl (25%; 7.7 M) 10.0 ml

FeCl₂ × 4 H₂O 1.5 g

ZnCl₂ 70.0 mg

MnCl₂ × 4 H₂O 100.0 mg

H₃BO₃ 6.0 mg

CoCl₂ × 6 H₂O 190.0 mg

CuCl₂ × 2 H₂O 2.0 mg

NiCl₂ × 6 H₂O 24.0 mg

Na₂MoO₄ × 2 H₂O 36.0 mg

Distilled water 990.0 ml

Vitamin solution:

Biotin 2.0 mg

Folic acid 2.0 mg

Pyridoxine  10.0 mg

Riboflavin  5.0 mg

Pantotenoic acid 5.0 mg

p-Aminobenzoic acid 5.0 mg

Thiamine-HCl 5.0 mg

Nicotinic acid 5.0 mg

Vitamin B₁₂ 0.1 mg

Lipoic acid 5.0 mg

Distilled water 1000.0 ml

Dissolve the ingredients (except formate, vitamin solution and sulfide) and flush medium with 100 % N₂ for 30 min. Add the sodium sulfide, adjust the pH to 8.8-9.0, dispense in Hungate tubes under N₂, and autoclave at 121°C 15 min. Before use add sodium formate and vitamin solution from a sterile, anaerobic stock solution (autoclave sodium formate 121°C 15 min and vitamin solution by filtration).

 

Updated 1.02.2024