244. SULFOBACILLUS MEDIUM

Solution A:

(NH₄)₂SO₄ 3.0 g

KCl 0.1 g

K₂HPO₄ 0.5 g

MgSO₄ × 7 H₂O 0.5 g

Ca(NO₃)₂ 0.01 g

Distilled water 680.0 ml

pH 2.0-2.2 (adjust with sulfuric acid)

Solution B:

FeSO₄ × 7 H₂O 44.2 g

Distilled water 300.0 ml

H₂SO₄ (10 N) 1.0 ml

Solution C:

Yeast extract (1% w/v in water) 20.0 ml

pH 1.9-2.4

After autoclaving at 121°C 15 min, combine the three solutions.

 

Updated 1.02.2024