247. RHODOBLASTUS MEDIUM

Yeast extract 0.1 g

Na2-succinate 1.0 g

KH2PO4 0.5 g

MgSO4 7 H2O 0.4 g

NaCl 0.4 g

NH4Cl 0.4 g

CaCl2 2 H2O 0.05 g

Trace element solution SL-6 (see below) 1.0 ml

Methanol 0.01-1%

Distilled water 1000.0 ml

pH 5.7

Trace element solution SL-6:

ZnSO4 7 H2O 0.1 g

MnCl2 4 H2O 0.03 g

H3BO3 0.3 g

CoCl2 6 H2O 0.2 g

CuCl2 2 H2O 0.01 g

NiCl2 6 H2O 0.02 g

Na2MoO4 2 H2O 0.03 g

Distilled water 1000.0 ml

Autoclave basal medium and trace element solution separately at 121C 15 min, methanol sterilize by filtration.

 

Updated 1.02.2024