248. PFENNIG'S MEDIUM

KH₂PO₄ 0.34 g

NH₄Cl 0.34 g

MgSO₄ × 7 H₂O 0.5 g

CaCl₂ × 2 H₂O 0.05 g

KCl 0.34 g

Trace element solution SLA (see below) 1.0 ml

Vitamin B₁₂ 20 µg

NaHCO₃ 1.5 g

Na₂S × 9 H₂O 0.4 g

Na₂S₂O₃ × 5 H₂O 0.5 g

NaCl 15.0 g

MgCl₂ × 6 H₂O 2.5 g.

pH 7.5

Trace element solution SLA:

FeCl₂ × 4 H₂O 1.8 mg

CoCl₂ × 6 H₂O 250 mg

NiCl₂ × 6 H₂O 10 mg

CuCl₂ × 2 H₂O 10 mg

MnCl₂ × 4 H₂O 70 mg

ZnCl₂ 100 mg

H₃BO₃ 500 mg

Na₂MoO₄ × 2 H₂O 30 mg

Na₂SeO₃ × 5 H₂O 10 mg

Distilled water 1000.0 ml

Autoclave basal medium and trace element solution separately at 121°C 15 min, vitamin B₁₂ sterilize by filtration.

 

Updated 1.02.2024