251. Chelativorans oligotrophicus MEDIUM

Na₄EDTA 1.0 g

Na₂HPO₄ × 12 H₂O 4.0 g

KH₂PO₄ 0.26 g

MgSO₄ × 7 H₂O 1.0 g

CaCl₂ × 2 H₂O 0.4 g

Trace element solution (see below) 2.0 ml

Vitamin solution (see below) 1.0 ml

Distilled water 1000.0 ml

pH 7.0

Trace element solution:

FeCl₂ × 4 H₂O 1.5 g

H₃BO₃ 60.0 mg

MnCl₂ × 4 H₂O 100.0 mg

CoCl₂ × 6 H₂O 120.0 mg

ZnCl₂ 70.0 mg

NiCl₂ × 6 H₂O 25.0 mg

CuCl₂ × 2 H₂O 15.0 mg

Na₂MoO₄ × 2 H₂O 25.0 mg

HCl, 0.05 molar 1000.0 ml

Vitamin solution:

Pyridoxine-HCl 100.0 mg

Thiamine-HCl × 2 H₂O 50.0 mg

Riboflavin 50.0 mg

Nicotinic acid 50.0 mg

Pantothenic acid 50.0 mg

p-Aminobenzoic acid 50.0 mg

Lipoic acid 50.0 mg

Nicotine amide 50.0 mg

Vitamin B₁₂ 50.0 mg

Biotin 20.0 mg

Folic acid 20.0 mg

Distilled water 1000.0 ml

Autoclave at 121°C 15 min, vitamin solution stir for some hours and sterilize by filtration.

 

Updated 1.02.2024