261. ROSEINATRONOBACTER MONICUS MEDIUM

NH₄Cl 0.4 g

KH₂PO₄ 0.5 g

MgCl₂ × 6 H₂O 0.2 g

Na₂SO₄ 0.5 g

NaNO₃ 0.4 g

NaCl 40.0 g

KCl 0.5 g

NaHCO₃ 10.0 g

Na₂CO₃ 5.0 g

Na₂S₂O₃ × 5 H₂O 1.0 g

Vitamin B₁₂ 10.0 μg

Sodium pyruvate 1.0 g

Sodium acetate 1.0 g

Peptone 1.0 g

Yeast extract 1.0 g

Trace element solution SL-10 1.0 ml

Distilled water 1000.0 ml

Trace element solution SL-10:

HCl (25%; 7.7 M) 10.0 ml

FeCl₂ × 4 H₂O 1.5 g

ZnCl₂ 70.0 mg

MnCl₂ × 4 H₂O 100.0 mg

H₃BO₃ 6.0 mg

CoCl₂ × 6 H₂O 190.0 mg

CuCl₂ × 2 H₂O 2.0 mg

NiCl₂ × 6 H₂O 24.0 mg

Na₂MoO₄ × 2 H₂O 36.0 mg

Distilled water 990.0 ml

The trace element solution preparation: FeCl₂ × 4 H₂O is dissolved firstly in HCl, and then is mixed with water and other salts are dissolved in the sequence indicated.

Autoclave the medium, trace element solution, NaHCO₃ and Na₂CO₃ separately at 121°C for 15 min and add to the medium. Add the vitamin B₁₂ solution from a filter-sterilized stock solution to the autoclaved, cooled medium.

Final pH 9.0 - 9.5

 

Updated 1.02.2024