269. THIOTHRIX MEDIUM

NH₄Cl 0.20 g

K₂HPO₄ 0.01 g

MgSO₄ × 7 H₂O 0.01 g

CaSO₄ (saturated solution) 20.0 ml

Trace element solution (see below) 5.0 ml

Na-acetate 0.10 g

Agar (if necessary) 12.0 g

Na₂S × 9 H₂O 10% (w/v) solution 3.0 ml

Distilled water 1000.0 ml

Adjust pH to 7.5 before autoclaving.

Trace element solution:

EDTA 0.20 g

FeSO₄ × 7 H₂O 0.70 g

ZnSO₄ × 7 H₂O 0.01 g

MnSO₄ × 4 H₂O 0.002 g

CuSO₄ × 5 H₂O 5.0 mkg

H₃BO₃ 10.0 mg

Co(NO₃)₂ 1.0 mg

Na₂MoO₄ × 2 H₂O 1.0 mg

Distilled water 1000.0 ml

Basal medium and Na₂S × 9 H₂O solution autoclave separately at 121°C 15 min.