278. MODIFIED S MEDIUM

KH₂PO₄ 0.14 g

MgSO₄ × 7 H₂O 0.4 g

CaCl₂ × 2 H₂O 0.1 g

NH₄Cl 0.25 g

KCl 0.36 g

NaCl 6.0

Yeast extract 0.1 g

Trace element solution according to Kevbrin and Zavarzin (see below) 1,0 ml

Vitamin solution according to Wolin et al. (see below) 1,0 ml.

Distilled water 1000.0 ml

Trace element solution according to Kevbrin and Zavarzin:

(NH₄)₂Fe(SO₄)₂ × 6 H₂O (Mohr's salt) 784.0 mg

HCl (concentrated) 5,0 ml

CoCl₂ × H₂O 238.0 mg

(NH₄)₂Ni(SO₄)₂ × 6 H₂O 395.0 mg

Na₂MoO₄ × H₂O 24.0 mg

Na₂WO₄ × 2 H₂O 33.0 mg

ZnSO₄ × 7 H₂O 144.0 mg

CuCl₂ × 2 H₂O 2.0 mg

Na₂SeO₄ 94.0 mg

H₃BO₃ 6.0 mg

MnCl₂ × 4 H₂O 99.0 mg

Distilled water 995.0 ml

Mohr's salt is dissolved firstly in concentrated HCl, and then is mixed with water and other salts are dissolved in the sequence indicated.

Vitamin solution according to Wolin et al.:

Biotin 20.0 mg

Folic acid 20.0 mg

Pyridoxine 0.1 mg

Riboflavin 50.0 mg

Pantotenoic acid 50.0 mg

p-Aminobenzoic acid 50.0 mg

Thiamine-HCl 50.0 mg

Nicotinic acid 50.0 mg

Vitamin B₁₂ 1.0 mg

Lipoic (thioctic) acid 50.0 mg

Distilled water 1000.0 ml

Autoclave at 121°C 15 min. Add filter-sterilization vitamine solution.