287. METHYLOCAPSA AUREA MEDIUM

NaNO₃ 200.0 mg

MgSO₄ × 6 H₂O 200.0 mg

CaCl₂ × 2 H₂O 40.0 mg

Fe-EDTA solution 3.0 ml

100mM phosphate buffer pH 5.8 10.0 ml

Trace elements solution (see below)1.0 ml

Distilled water 986.0 ml

Trace elements solution:

EDTA 5.0 g

CuCl₂ × 2 H₂O 0.1 g

FeSO₄ × 7 H₂O 2.0 g

ZnSO₄ × 7 H₂O 0.1 g

NiCl₂ × 6 H₂O 0.02 g

CoCl₂ × 6 H₂O 0.2 g

Na₂MoO₄ 0.03 g

Distilled water 1000.0 ml

FeEDTA solution:

FeSO₄ × 7 H₂O 1.54 g

Na₂EDTA 2.06 g

Distilled water 1000.0 ml

100mM phosphate buffer:

K₂HPO₄ × 3 H₂O 1.71 g

KH₂PO₄ 12.58 g

Distilled water 1000.0 ml

Autoclave at 121°C for 15 min. Final pH 5.0-5.8. 20-30% methane is added to the gas phase. The strain should be grown with shaking.