289. MEDIUM FOR ECTOTHIORHODOSPIRA

KH₂PO₄ 0.5 g

CaCl₂ × 2H₂O 0.05 g

NH₄Cl 0.8 g

MgCl₂ × 6 H₂O 0.1 g

NaCl 30.0 g

Na₂SO₄ 20.0 g

Na₂CO₃ 6.0 g

Trace element solution (see below) 1.0 ml

Yeast extract 0.5 g

Na-succinate 1.0 g

Na₂S × 9 H₂O 1.0 g

Distilled water 800.0 ml

Adjust pH to 8.5

Trace element solution:

FeCl₂ × 4 H₂O 1.8 g

CoCl₂ × 6 H₂O 250.0 mg

NiCl₂ × 6 H₂O 10.0 mg

CuCl₂ × 2 H₂O 10.0 mg

MnCl₂ × 4 H₂O 70.0 mg

ZnCl₂ 100.0 mg

H₃BO₃ 500 mg

Na₂MoO₄ × 2 H₂O 30.0 mg

Na₂SeO₃ × 5 H₂O 10.0 mg

Distilled water 1000.0 ml

For dissolving adjust pH to about 3 with 1 N HCl

Vitamin solution:

Biotin 10.0 mg

Nicotinamide 35.0 mg

Thiamine-HCl × 2 H₂O 30.0 mg

p-Aminobenzoic acid 20.0 mg

Pyridoxal-HCl 10.0 mg

Ca-pantothenate 10.0 mg

Vitamin B₁₂ 5.0 mg

Distilled water 100.0 ml

Bicarbonate solution:

NaHCO₃ 14,0 g

H₂O 200.0 ml

Sterilize at 121°C for 15 minutes in screw-capped bottles. After sterilization add 1 ml/l filter-sterilized vitamin solution and 200 ml of filter-sterilized bicarbonate solution