30. AZOTOBACTER MEDIUM 1

Glucose 5.0 g

Mannitol 5.0 g

CaCl₂ × 2 H₂O 0.1 g

MgSO₄ × 7 H₂O 0.1 g

Na₂MoO₄ × 2 H₂O 5.0 mg

K₂HPO₄ 0.9 g

KH₂PO₄ 0.1 g

FeSO₄ × 7 H₂O 0.01 g

CaCO₃ 5.0 g

Agar 15.0 g

Distilled water 950.0 ml

pH 7.3

Autoclave at 121°C for 15 min.

Sterilize glucose and mannitol separately (in 50.0 ml H₂O) and add to the medium after autoclaving.