300. TELMATOSPIRILLUM MEDIUM

K₂HPO₄ 0.25 g

KH₂PO₄ 1.0 g

(NH₄)₂SO₄ 1.0 g

CaCl₂ 0.1 g

MgSO₄ 0.4 g

Na-EDTA 0.01 g

FeCl₃ × 6 H₂O 1.0 mg

KI 0.2 mg

CoCl₂ × 6 H₂O 0.2 mg

MnCl₂ × 4 H₂O 0.8 mg

ZnSO₄ 0.8 mg

H₃BO₂ 0.1 mg

Na₂MoO₄ × 2 H₂O 0.1 mg

CuCl₂ 0.1 mg

NiCl₂ × 6 H₂O 0.2 mg

Na-citrate 2.0 g

Distilled water 1000.0 ml

Prepare the medium anaerobically under nitrogen. Adjust to pH 5.5–6.5 with 1M H₃PO₄. Dispense into serum bottles or Hungate tubes (under a nitrogen atmosphere) that can be sealed with rubber stoppers. Sterilize by autoclaving at 121°C for 15 min.