310. CLOSTRIDIUM THERMOCELLUM MEDIUM

(NH₄)₂SO₄ 1.3 g

MgCl₂ × 6 H₂O 2.6 g

KH₂PO₄ 1.43 g

K₂HPO₄ 5.5 g

CaCl₂ × 2 H₂O 0.13 g

Na₂-ß-glycerol phosphate × 4 H₂O 6.0 g

FeSO₄ × 7 H₂O solution (0.1% w/v in 0.1 N H₂SO₄) 1.1 ml

L-Glutathione reduced 0.25 g

Yeast extract 4.5 g

Na-resazurin solution (0.1% w/v) 0.5 ml

Cellobiose 5.0 g

Distilled water 1000.0 ml

Dissolve ingredients except cellobiose, sparge medium with 80% N₂ and 20% CO₂ gas mixture for 30 – 45 min to make it anoxic. Then adjust pH to 7.0 - 7.2, distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Cellobiose is added to the sterile medium from an anoxic 10% (w/v) stock solution prepared under 100% N₂ gas and sterilized by filtration.

 

Updated 1.02.2024