315. FUCHSIELLA MEDIUM

KH₂PO₄ 0.2 g

MgCl₂ × 6 H₂O 0.1 g

NH₄Cl 0.5 g

KCl 0.2 g

NaCl 60.0 g

Na₂CO₃ 68.3 g

NaHCO₃ 38.3 g

Trace element solution SL-4 (see below) 1.0 ml

Resazurin 0.5 mg

Vitamin solution (see below) 10.0 ml

Yeast extract 0.05 g

Ethanol (50% v/v) 10.0 ml

Na₂S × 9 H₂O 1.0 g

NaNO₃ 0.85 g

Distilled water 1000.0 ml

Trace element solution SL-4:

EDTA 0.5 g

FeSO₄ × 7 H₂O 0.2 g

Trace element solution SL-6 (see below) 100.0 ml

Distilled water 900.0 ml

Trace element solution SL-6:

ZnSO₄ × 7 H₂O 0.1 g

MnCl₂ × 4 H₂O 0.03 g

H₃BO₃ 0.3 g

CoCl₂ × 6 H₂O 0.2 g

CuCl₂ × 2 H₂O 0.01 g

NiCl₂ × 6 H₂O 0.02 g

Na₂MoO₄ × 2 H₂O 0.03 g

Distilled water 1000.0 ml

Vitamin solution:

Biotin 2.0 mg

Folic acid 2.0 mg

Pyridoxine-HCl 10.0 mg

Thiamine-HCl × 2 H₂O 5.0 mg

Riboflavin 5.0 mg

Nicotinic acid 5.0 mg

D-Ca-pantothenate 5.0 mg

Vitamin B₁₂ 0.1 mg

p-Aminobenzoic acid 5.0 mg

Lipoic acid 5.0 mg

Distilled water 1000.0 ml

pH 8.8- 9.5

Dissolve ingredients except the sodium carbonate, sodium bicarbonate, ethanol, and sodium sulfide. Boil medium for a few minutes and cool to room temperature while flushing with N₂. Add and dissolve sodium carbonate, sodium bicarbonate and sodium sulfide while gassing the head space only. Dispense and autoclave under N₂. Before use add 10 ml/l (v/v) of 50% (v/v) ethanol solution (flushed and autoclaved under N₂).

 

Updated 1.02.2024