33. PEPTONE MEAT AGAR WITH 0.2% UREA
Peptone 10.0 g
NaCl 5.0 g
Beef extract 3.0 g
Agar 20.0 g
Distilled water 1000.0 ml
Do not adjust pH; pH rises to about 8 due to heat degraded urea.
Autoclave at 121°C for 15 min.
10 ml filter-sterilized 20% urea solution is added aseptically after autoclaving to 1000 ml cooled, molten, agar. The medium is then immediately dispensed aseptically.