33. PEPTONE MEAT AGAR WITH 0.2% UREA

Peptone 10.0 g

NaCl 5.0 g

Beef extract 3.0 g

Agar 20.0 g

Distilled water 1000.0 ml

Do not adjust pH; pH rises to about 8 due to heat degraded urea.

Autoclave at 121°C for 15 min.

10 ml filter-sterilized 20% urea solution is added aseptically after autoclaving to 1000 ml cooled, molten, agar. The medium is then immediately dispensed aseptically.