332. THERMOGUTTA MEDIUM

KH₂PO₄ 0.33 g

MgCl₂ × 6 H₂O 0.33 g

CaCl₂ × 2 H₂O 0.33 g

NH₄Cl 0.33 g

KCl 0.33 g

NaCl 6.0

Yeast extract 0.2 g

Xanthan 5.0 g

KNO₃ 2.0 g

NaHCO₃ 2.0 g

Trace element solution (see below) 1.0 ml

Vitamin solution (see below) 1.0 ml

Trace element solution:

Fe(NH₄)₂(SO₄)₂ × 6 H₂O (Mohr's salt) 784.0 mg

CoCl₂ × H₂O 238.0 mg

Ni(NH₄)₂(SO₄)₂ × 6 H₂O 395.0 mg

Na₂MoO₄ × 2 H₂O 24.0 mg

Na₂WO₄ × 2 H₂O 33.0 mg

ZnSO₄ × 7 H₂O 144.0 mg

CuCl₂ × 2 H₂O 2.0 mg

Na₂SeO₄ 94.0 mg

H₃BO₃ 6.0 mg

MnCl₂ × 4 H₂O 99.0 mg

Mohr's salt is dissolved firstly in concentrated HCl, and then is mixed with water and other salts are dissolved in the sequence indicated.

Vitamin solution:

Biotin 20.0 mg

Folic acid 20.0 mg

Pyridoxine 10.0 mg

Riboflavin  50.0 mg

Pantotenoic acid 50.0 mg

p-Aminobenzoic acid 50.0 mg

Thiamine-HCl 50.0 mg

Nicotinic acid 50.0 mg

Vitlamin B₁₂ 1.0 mg

Lipoic acid 50.0 mg

Distilled water 1000.0 ml

The media was boiled and cooled under the flow of oxygen-free gases (CO₂ or N₂) to render the media anaerobic and finally heat-sterilized at 121°C for 60 min. No reducing agents were added.

Updated 01.02.2024