340. DISSULFURIMICROBIUM MEDIUM

KH₂PO₄ 0.33 g

NH₄Cl 0.33 g

KCl 0.33 g

CaCl₂ × 2 H₂O 0.33 g

MgCl₂ × 6 H₂O, 0.33 g

NaHCO₃ 2.0 g

Vitamin solution (see below) 10.0 ml

Trace element solution (see below) 1.0 ml

Ferrihydrite

Distilled water 990.0 ml

Vitamin solution:

Biotin 2.0 mg

Folic acid 2.0 mg

Pyridoxine (B₂) 10.0 mg

Riboflavin (B₁) 5.0 mg

Pantotenoic acid 5.0 mg

p-Aminobenzoic acid 5.0 mg

Thiamine-HCl 5.0 mg

Nicotinic acid 5.0 mg

Vitamin B₁₂ 0.1 mg

Lipoic acid 5.0 mg

Distilled water 1000.0 ml

pH 7.2-7.4

Trace element solution:

Na₂-EDTA × 2 H₂O 5.2 g

FeCl₂ × 4 H₂O 1.5 g

ZnCl₂ 70.0 mg

MnCl₂ × 4 H₂O 100.0 mg

H₃BO₃ 6.0 mg

CoCl₂ × 6 H₂O 190.0 mg

CuCl₂ × 2 H₂O 2.0 mg

NiCl₂ × 6 H₂O 24.0 mg

Na₂MoO₄ × 2 H₂O 36.0 mg

Distilled water 1000.0 ml

Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000.0 ml. The pH of the autoclaved medium was 6.7–6.8. Medium (10.0 ml) was dispensed into 17 ml Hungate tubes; the headspace was filled with CO₂ (100 %).

 

Updated 1.02.2024