346. Thermaerobacter baikalensis medium

Peptone 1.25 g

Yeast extract 0.25 g

Fe(III) citrate 0.025 g

NaCl 5.0 g

MgCl₂ × 6 H₂O 1.0 g

Na₂SO₄ 0.8 g

CaCl₂ × 2 H₂O 0.45 g

KCl 0.1 g

NaHCO₃ 0.4 g

KBr 0.02 g

SrCl₂ × 6 H₂O 0.03 g

H₃BO₃ 0.02 g

Agar 20.0 g

Trace element solution (see below) 1.0 ml

Distilled water 1000.0 ml. Autoclave at 121°C for 15 min.

Final pH 7.0

Trace element solution:

Na-silicate 4.0 g

NaF 2.4 g

NH₄NO₃ 1.6 g

Na₂HPO₄ 8.0 g

Distilled water 1000.0 ml. Autoclave the trace element solution separately at 121°C for 15 min and add to the medium.

 

Updated 01.02.2024