349. Tautongia SOCIABILIS MEDIUM

KH₂PO₄ 0.33 g

MgCl₂ × 6 H₂O 0.33 g

CaCl₂ × 2 H₂O 0.33 g

NH₄Cl 0.33 g

KCl 0.33 g

Xylose or trehalose 1.0 g

Trace element solution (see below) 1.0 ml

Vitamin solution (see below) 1.0 ml

Distilled water 1000.0 ml

Autoclave base medium, KH₂PO₄ (in 10.0 ml distilled water) and trace element solution at 121°C for 15 min. Vitamin solution sterilize by filtration. Adjust pH to 7.5-7.7.

Trace element solution:

(NH₄)₂Fe(SO₄)₂ × 6 H₂O (Mohr's salt) 784.0 mg

HCl (concentrated) 5.0 ml

CoCl₂ × H₂O 238.0 mg

(NH₄)₂Ni(SO₄)₂ × 6 H₂O 395.0 mg

Na₂MoO₄ × 2 H₂O 24.0 mg

Na₂WO₄ × 2 H₂O 33.0 mg

ZnSO₄ × 7 H₂O 144.0 mg

CuCl₂ × 2 H₂O 2.0 mg

Na₂SeO₄ 94.0 mg

H₃BO₃ 6.0 mg

MnCl₂ × 4 H₂O 99.0 mg

Distilled water 995.0 ml

Mohr's salt is dissolved firstly in concentrated HCl, then is mixed with 1000.0 ml distilled water and other salts are dissolved in the sequence indicated.

Vitamin solution:

Biotin 20.0 mg

Folic acid 20.0 mg

Pyridoxine 100.0 mg

Riboflavin 50.0 mg

Pantotenoic acid 50.0 mg

p-Aminobenzoic acid 50.0 mg

Thiamine-HCl 50.0 mg

Nicotinic acid 50.0 mg

Vitamin B₁₂ 1.0 mg

Lipoic acid 50.0 mg

Distilled water 1000.0 ml

Adjust pH to 5.9.

After autoclaving at 121°C for 15 min add sterile stock solution NaOH (0.5 M, 3.2 ml/l medium) to readjust pH to 6.8. Add the vitamin solution and trace element solution from sterile stock solutions.

 

Updated 1.02.2024