354. BACTO MARINE BROTH (DIFCO 2216)

Bacto peptone 5.0 g

Bacto yeast extract 1.0 g

Fe(III) citrate 0.1 g

NaCl 80.0 g

MgCl₂ (anhydrous) 5.9 g

Na₂SO₄ 3.24 g

CaCl₂ × 2 H₂O 1.8 g

KCl 0.55 g

NaHCO₃ 0.16 g

KBr 0.08 g

SrCl₂ 34.0 mg

H₃BO₃ 22.0 mg

Na-silicate 4.0 mg

NaF 2.4 mg

NH₄NO₃ 1.6 mg

Na₂HPO₄ 8.0 mg

Distilled water 1000.0 ml C.

Final pH should be 7.6 ± 0.2 at 25 °C

If using the complete medium from Difco add 37.4 g per liter water + 6% NaCl.

 

Updated 1.02.2024