375. Methylomicrobium buryatense MEDIUM

KH₂PO₄ × 12 H₂O 0.5 g

KNO₃ 0.5 g

MgSO₄ × 7 H₂O 0.4 g

NaCl 5.0 g

NaHCO₃ (1M) 25.0 ml

Na₂CO₃ (1M) 5.0 ml

Distilled water 1000.0 ml

pH 9.0–9.5

Trace element solution (see below) 1 ml

Trace elements solution:

EDTA 5.0 g

CuCl₂ × 2 H₂O 0.1 g

FeSO₄ × 7 H₂O 2.0 g

ZnSO₄ × 7 H₂O 0.1 g

NiCl₂ × 6 H₂O 0.02 g

CoCl₂ × 6 H₂O 0.2 g

Na₂MoO₄ 0.03 g

Distilled water 1000.0 ml

Prepare the medium without the NaHCO₃ and Na₂CO₃. Basal medium, trace element, NaHCO₃ (1M) and Na₂CO₃ (1M) sterilize separately at 121°C 15 min. When preparing liquid media cool the mineral salts solution, NaHCO₃ and Na₂CO₃ solutions to room temperature before mixing. When preparing agar add 2.0 % agar to the mineral salt solution and autoclave. Cool NaHCO₃ and Na₂CO₃ stock solutions and agar to 50-55°C before mixing.

 

Updated 12.07.2021