376. Mineral medium with gelatin

NaCl 10.0 g

KH₂PO₄ 0.14 g

KCl 0.36 g

MgCl₂ × 6 H₂O 0.4 g

CaCl₂ × 2 H₂O 0.05 g

NH₄Cl 0.25 g

3–(N–Morpholino)propanesulfonic acid (MOPS) buffer 2.09 g

Gelatin 1.0 g

Distilled water 1000.0 ml

Adjust the pH to 7.5 if needed with NaOH/HCl. Autoclave at 121°C for 15 min.

 

Updated 12.07.2021