381. ALCALICAULIS SATELITICUS MEDIUM

NaCl 42.0 g

NaHCO₃ 24.0 g

KH₂PO₄ 0.3 g

MgSO₄ × 7 H₂O 0.12 g

CaCl₂ × 2 H₂O 0.03 g

Trypton 2.0 g

Trace element solution (see below) 1.0 ml

Distilled water 1000.0 ml

Trace element solution:

FeSO₄ × 7 H₂O 0.6 mg

CoCl₂ × 6 H₂O 0.12 mg

Ni(NH₄)₂(SO₄)₂ × 6 H₂O 0.9 mg

MnCl₂ × 4 H₂O 0.1 mg

ZnSO₄ × 7 H₂O 0.14 mg

H₃BO₃ 0.03 mg

AlK(SO₄)₂ × 12 H₂O 0.24 mg

Na₂MoO₄ × 2 H₂O 0.02 mg

Na₂WO₄ × 2 H₂O 0.03 mg

Na₂SeO₃ × 5 H₂O 0.02 mg

CuCl₂ × 2 H₂O 0.02 mg

Distilled water 1000.0 ml

Prepare the medium without NaHCO₃. Basal medium, trace element solution and NaHCO₃ sterilize separately at 121°C for 15 min. When preparing liquid media cool the mineral salts solution and NaHCO₃ to room temperature before mixing. When preparing agar add 2.0 % agar to the mineral salt solution and autoclave. Cool the NaHCO₃ stock solution and agar to 50-55°C before mixing.

Updated 11.08.2021