CaCl2 2 H2O 0.12 g

MgSO4 x 7 H2O 0.19 g

KH2PO4 0.45 g

K2HPO4 0.45 g

NaCl 0.9 g

(NH4)2SO4 0.9 g

Yeast extract 2.0 g

Trypticase peptone 2.0 g

Na-resazurin solution (0.1% w/v) 0.5 ml

L-Cysteine-HCl H2O 1.0 g

Na2CO3 2.5 g

D-Glucose 2.0 g

Distilled water 1000.0 ml

Dissolve ingredients (except cysteine, carbonate and glucose), adjust pH to 7.0 and sparge medium with 100% CO2 gas for 10 15 min to make it anoxic. Add the cysteine and carbonate, then equilibrate the medium with the CO2 gas to pH 7.0. Distribute medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Thereafter, add glucose from an anoxic stock solution prepared under 100% N2 gas atmosphere and sterilized by filtration.


Updated 11.08.2021