389. TRICHOCOCCUS MEDIUM

CaCl₂ × 2 H₂O 0.12 g

MgSO₄ x 7 H₂O 0.19 g

KH₂PO₄ 0.45 g

K₂HPO₄ 0.45 g

NaCl 0.9 g

(NH₄)₂SO₄ 0.9 g

Yeast extract 2.0 g

Trypticase peptone 2.0 g

Na-resazurin solution (0.1% w/v) 0.5 ml

L-Cysteine-HCl × H₂O 1.0 g

Na₂CO₃ 2.5 g

D-Glucose 2.0 g

Distilled water 1000.0 ml

Dissolve ingredients (except cysteine, carbonate and glucose), adjust pH to 7.0 and sparge medium with 100% CO₂ gas for 10 – 15 min to make it anoxic. Add the cysteine and carbonate, then equilibrate the medium with the CO₂ gas to pH 7.0. Distribute medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Thereafter, add glucose from an anoxic stock solution prepared under 100% N₂ gas atmosphere and sterilized by filtration.

 

Updated 11.08.2021