395. FRIGORIGLOBUS TUNDRICOLA MEDIUM

Glucose 0.5 g

Yeast extract 0.25 g

Peptone 0.25 g

N-acetylglucosamine 0.5 g

KH₂PO₄ 0.1 g

MgSO₄ × 7 H₂O 0.1 g

NH₄NO₃ 0.1 g

CaCl₂ × 6 H₂O 0.02 g

Distilled water 1000.0 ml (800.0 ml for solid medium)

pH 5.8-6.0

Autoclave at 121°C for 15 min

For solid medium 8.0 g/l Phytagel in 200.0 ml distilled water (mix before autoclaving), autoclave separately. Gelrite gets solid below 80°C after autoclaving and in general polymerization starts fast after mixing with media.

Updated 17.07.2021