400. THIOTHRIX MEDIUM

NH₄Cl 0.3 g

CaCl₂ 0.03 g

KH₂PO₄ 0.01 g

K₂HPO₄ 0.022 g

Na₂HPO₄ × 7 H₂O 0.035 g

MgSO₄ × 7 H₂O 0.05 g

FeCl₃×6H₂O 0.002 g

NaNO₃ 0.30 g

Peptone 0.2 g

Na-acetate (10%) 2.5 ml

Na-lactate (10%) 2.5 ml

NaHCO₃ (10%) 5.0 ml

Na₂S₂O₃ × 5 H₂O (10%) 10.0 ml

Vitamin solution (see below) 1.0 ml

Trace element solution according to Hogland: (see below) 1.0 ml

Distilled water 1000.0 ml

Vitamin solution:

Biotin 200.0 mg

Folic acid 20.0 mg

Pyridoxine-HCl 100.0 mg

Thiamine-HCl 50.0 mg

Riboflavin 100.0 mg

Nicotinic acid 50.0 mg

DL-Pantothenic acid 50.0 mg

Vitamin B₁₂ 1.0 mg

p-Aminobenzoic acid 50.0 mg

Distilled water 1000.0 ml

Trace element solution according to Hogland:

EDTA 5.0 g

FeSO₄ × 7 H₂O 2.0 g

ZnSO₄ × 7 H₂O 100.0 mg

MnCl₂ × 4 H₂O 30.0 mg

H₃BO₃ 300.0 mg

CoCl₂ × 6 H₂O 200.0 mg

CuCl₂ 10.0 mg

NiCl₂ × 6 H₂O 20.0 mg

Na₂MoO₄ × 2 H₂O 20.0 mg

Distilled water 1000.0 ml

pH 7.5 (adjust with 1% HCl)

To prepare the trace element solution, preliminarily acidify water to pH 3.0-4.0 with HCl. Sterilize thiosulfate, acetate, lactate, bicarbonate, trace elements and vitamins separately and add to the main medium prior to inoculation. Sterilize the vitamin solution by filtration, others solutions and base medium by autoclaving at 121°C for 15 min.

Updated 10.08.2021