402. PROSTHECODIMORPHA STALEYI MEDIUM (PSM)

Glucose 0.3 g

Na₂HPO₄ 0.071 g

(NH₄)₂SO₄ 0.25 g

Yeast extract 0.05 g

Peptone 0.1 g

Vitamin solution (see below) 10.0 ml

Hutner's salt solution (see below) 20.0 ml

Distilled water 970.0 ml

Vitamin solution :

Biotin 2.0 mg

Folic acid 2.0 mg

Thiamine-HCl 5.0 mg

D-Ca- Panthothenate 5.0 mg

Vitamin B₁₂ 0.1 mg

Riboflavin 5.0 mg

Distilled water 1000.0 ml

Hutner’s Salt Solution:

Nitrilotriacetic acid 10.0 g

MgSO₄ × 7 H₂O 29.7 g

CaCl₂ × 2 H₂O 3.335 g

FeSO₄ × 7 H₂O 99.0 mg

(NH₄)₆Mo₇O₂₄ × 4 H₂O 9.25 mg

"Metals 44" 50.0 ml

Distilled water 1000.0 ml

"Metals 44":

ZnSO₄ × 7 H₂O 1.095 g

FeSO₄ × 7 H₂O 0.5 g

EDTA-Na 0.25 g

MnSO₄ × H₂O 0.154 g

CuSO₄ × 5 H₂O 39.2 mg

Co(NO₃)₂ × 6 H₂O 24.8 mg

Na₂B₄O₇ × 10 H₂O 17.7 mg

Distilled water 100.0 ml