407. PSEUDOALTEROMONAS TELLURITIREDUCENS MEDIUM (PTM)

MgSO₄ × 7 H₂O 0.5 g

KH₂PO₄ 0.3 g

NH₄Cl 0.3 g

CaCl₂ × 2 H₂O 0.05 g

Na-acetate × 3 H₂O 10.0 g

Yeast extract 0.5 g

Casamino acids 0.5 g

NaCl 15.0 g

Trace elements solution (see below) 2.0 ml

Vitamin solution (see below) 2.0 ml

Distilled water 1000.0 ml

Trace elements solution:

MnCl₂ × 4 H₂O 0.3 g

FeSO₄ × 7 H₂O 0.03 g

Distilled water 1000.0 ml

Vitamin solution:

Biotin 80 mg

Thiamine-HCl 400.0 mg

Nicotinic acid 400.0 mg

Vitamin B₁₂ 20.0 μg

Distilled water 1000.0 ml

After autoclaving at 121°C for 15 min adjust to pH 7.8 with NaHCO₃. Add the vitamin solution and trace elements solution from sterile stock solutions.