414. METHYLOCAPSA ACIDIPHILA MEDIUM (MAM)

KH₂PO₄ 100.0 mg

MgSO₄ × 7 H₂O 50.0 mg

CaCl₂ × 2 H₂O 10.0 mg

Trace element solution (see below) 1.0 ml

Distilled water 1000.0 ml

Trace element solution:

EDTA 5.0 g

CuCl₂ × 5 H₂O 0.10 g

FeSO₄ × 7 H₂O 2.0 g

ZnSO₄ × 7 H₂O 0.1 g

NiCl₂ × 6 H₂O 0.02 g

CoCl₂ × 6 H₂O 0.2 g

Na₂MoO₄ 0.03 g

Distilled water 1000.0 ml

Final pH 4.0-4.5.

The medium is fairly weakly buffered so the pH should be checked before and after autoclaving. In the early stage of growth the strain may form aggregates which break as the culture ages. 10-30% methane is added to the gas phase. Adding 100 mg/litre KNO₃ enhances growth.

The strain should be grown with shaking.