415. MEDIUM FOR ECTOTHIORHODOSYNUS (MERh)

KH₂PO₄ 0.5 g

NH₄Cl 0.5 g

NaCl 20.0 g

MgCl₂ × 6 H₂O 0.2 g

CaCl₂ 0.1 g

Na₂CO₃ 5.0 g

NaHCO₃ 5.0 g

Sodium acetate 1.0 g

Yeast extract 0.1 g

Na₂S₂O₃ × 5 H₂O 0.5 g

Na₂S × 9 H₂O 0.1 g

Trace element solution SL6 (see below) 1.0 ml

Vitamin B₁₂ 20.0 μg

Distilled water 1000.0 ml

Trace element solution SL-6:

ZnSO₄ × 7 H₂O 0.1 g

MnCl₂ × 4 H₂O 0.03 g

H₃BO₃ 0.3 g

CoCl₂ × 6 H₂O 0.2 g

CuCl₂ × 2 H₂O 0.01 g

NiCl₂ × 6 H₂O 0.02 g

Na₂MoO₄ 0.03 g

Distilled water 1000.0 ml

Prepare the NH₄Cl, CaCl₂, carbonate, bicarbonate and sulfide as a single sterile stock solution and add to the sterile medium after autoclaving. The pH should be adjust to 9-9.5.