437. MODIFIED MARINE MEDIUM (MMM)

NaCl 20.0 g

K₂HPO₄ 0.15 g

CaCl₂ × 2 H₂O 0.15 g

NH₄Cl 0.25 g

MgSO₄ × 7 H₂O 3.45 g

MgCl₂ × 6 H₂O 2.75 g

KCl 0.33 g

NiCl₂ × 6 H₂O 0.5 mg

Na₂SeO₄ × 5 H₂O 0.5 mg  

Fe(NH₄)₂(SO₄)₂ × 6 H₂O 0.01 g

Trace mineral solution (see below) 10.0 ml

Vitamin solution (see below) 5.0 ml

Yeast extract 0.10 g  

Trypticase peptone 1.0 g

Distilled water 1000.0 ml

Trace mineral solution (Kevbrin & Zavarzin, 1992):

(NH₄)₂SO₄FeSO₄ × 6 H₂O 784.0 mg

MnCl₂ × 4 H₂O 99.0 mg

CoCl₂ × 6 H₂O 238.0 mg

(NH₄)₂SO₄NiSO₄ × 6 H₂O 395.0 mg

Na₂MoO₄ × H₂O 24.0 mg

Na₂WO₄ × 2 H₂O 33.0 mg

ZnSO₄ × 7 H₂O 144.0 mg

CuCl₂ × 6 H₂O 238.0 mg

Na₂SeO₄ × 5 H₂O 94.0 mg

H₃BO₃ 6.0 mg

Distilled water 1000.0 ml

Vitamin solution:

Biotin 2.0 mg

Folic acid 2.0 mg

Pyridoxine-HCl 10.0 mg

Thiamine-HCl 5.0 mg

Riboflavin 5.0 mg

Nicotinic acid 5.0 mg

Ca-D-pantothenate 5.0 mg

Vitamin B₁₂ 0.10 mg

p-Aminobenzoic acid 5.0 mg

(±)-α-Lipoic acid 5.0 mg

Distilled water 1000.0 ml