449. PROSTHECOCHLORIS MARINA MEDIUM (PMM)

KH₂PO₄ 0.5 g

NH₄Cl 0.5 g

NaCl 15.0 g

MgCl₂ × 6 H₂O 0.2 g

CaCl₂ 0.1 g

NaHCO₃ 3.0 g

Sodium acetate 0.5 g

Yeast extract 0.1 g

Na₂S₂O₃ × 5 H₂O 1.0 g

Na₂S × 9 H₂O 0.7 g

Trace element solution SL6 (see below) 1.0 ml

Vitamin solution 1.0 ml (see below)

Distilled water 1000.0 ml

Trace element solution SL-6:

ZnSO₄ × 7 H₂O 0.1 g

MnCl₂ × 4 H₂O 0.03 g

H₃BO₃ 0.3 g

CoCl₂ × 6 H₂O 0.2 g

CuCl₂ × 2 H₂O 0.01 g

NiCl₂ × 6 H₂O 0.02 g

Na₂MoO₄ 0.03 g

Distilled water 1000.0 ml

Vitamin solution:

Biotin 20.0 mg

Folic acid 20.0 mg

Pyridoxine 100 mg

Riboflavin 50.0 mg

Pantotenoic acid 50.0 mg

p-Aminobenzoic acid 50.0 mg

Thiamine-HCl 50.0 mg

Nicotinic acid 50.0 mg

Vitamin B₁₂ 1.0 mg

Lipoic acid 50.0 mg

Distilled water 1000.0 ml

Prepare sulfide as a single sterile stock solution and add to the sterile medium after autoclaving. The pH should be adjust to 7.0-7.2.