459. ACIDITHIOBACILLUS FERROOXIDANS MEDIUM (AFM)

Solution A:

(NH₄)₂SO₄ 3.0 g

KCl 0.1 g

K₂HPO₄ 0.5 g

MgSO₄ × 7H₂O 0.5 g

Ca(NO₃)₂ 0.01 g

Distilled water 700 ml

Solution B:

FeSO₄ × 7 H₂O 44.22 g

10 N H₂SO₄ 4.5 ml

Distilled water 295.5 ml

Autoclave separately at 111°C for 30 min, cool to room temperature and mix.