460. MEDIUM FOR METHYLOMONAS SP. (MMsp)

KNO₃ 0.5 g

MgSO₄ × 7 H₂O 0.5 g

CaCl₂ 0.1 g

Na₂HPO₄ × 12 H₂O 1.5 g

KH₂PO₄ 0.7 g

Trace element solution (see below) 1.0 ml

Distilled water 1000.0 ml

Trace elements solution:

EDTA 5.0 g

FeSO₄ × 7 H₂O 2.0 g

ZnSO₄ × 7 H₂O 0.1 g

MnCl₂ × 4 H₂O 0.03 g

CoCl₂ × 6 H₂O 0.2 g

CuSO₄ × 5 H₂O 0.1 g

NiCl₂ × 6 H₂O 0.02 g

Na₂MoO₄ 0.03 g

Distilled water 1000.0 ml

pH 6.7-7.1

Autoclave at 121°C 15 min.

Cultivation under mixture of methane and air (30:70).

Updated 08.11.2022