474. MEDIUM FOR HALAMONAS DECHROMATICANS (HDM)

Solution 1

Na₂CO₃ 76.0 g

NaHCO₃ 12.82 g

NaCl 69.0 g

K₂HPO₄ 1.0 g

Distilled water 900,0 ml.

Solution 2

Na acetate 2,0 g

NaNO₃ 1.0 г

Distilled water 100,0 ml.

MgSO₄ 10% 1 ml.

NH₄Cl 10% 1 ml.

Yeast extract 10% 1 ml.

Trace element solution according to Pfennig (see below) 2,0 ml.

Trace element solution according to Pfennig:

EDTA 1,5 g

Trace element solution according to Hogland (see below) 6,0 ml.

FeSO₄ × 7 H₂O 0.2 g

MnCl₂ × 4 H₂O 0.02 g

ZnSO₄ × 7 H₂O 0.1 g

Distilled water 1000,0 ml.

Trace element solution according to Hogland:

EDTA 5,0 g

FeSO₄ × 7 H₂O 2.0 g

ZnSO₄ × 7 H₂O 100.0 ml

MnCl₂ × 4 H₂O 30.0 mg

H₃BO₃ 300.0 мг

CoCl₂ × 6 H₂O 200.0 mg

CuCl₂ × 2 H₂O 10.0 mg

NiCl₂ × 6 H₂O 20.0 mg

Na₂MoO₄ × 2 H₂O 20.0 mg

Distilled water 1000.0 ml

Autoclave solutions 1, 2, MgSO₄, NH₄Cl, yeast extract and trace element solution separately at 121 C for 15 min and mix aseptically.

Updated: 19.11.2023